Studies of Bioactive Metabolites from Endophytic Bacterial Isolate

The microbial source, variety of producing species, functions and an assortment of bioactivities of metabolites. The probable numbers of metabolites may be discovered in the future, the problems of dereplication of recently isolated compounds as well as the new trends and prediction of the research are also discussed. Ten isolates were isolated from different plant parts (root, stem, leaves) of Adhathoda beddomei (adosa), a medicinal plant. Among ten isolates, one isolate exhibited highest antimicrobial activity against gram positive and gram negative bacteria. Recognition of endophytes was done on the basis of morphological characteristics. The antimicrobial activity was tested against Escherichia coli. Pseudomonas aeroginosa, Staphylococcus aureus, Penicillium chrysogenum, Lactobacillus lactis, Bacillus subtilis and Candida albican. Minimum inhibitory concentration (MIC) of crude extract performed against microorganisms was determined. The controlling parameters the biosynthetic process of the antimicrobial agent formation including; different pH values, temperatures, incubation period, and different carbon source. NMR, FTIR, element and mass analysis of fractionated crude extract was carried out for functional group and experimental formula determination.